Anti-NGAL (monkey, neutrophil gelatinase-associated lipocalin)
Mouse monoclonal antibody
Cat.No. ABS 038-23
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3).
Recombinant human NGAL adsorbed onto aluminum hydroxide gel
ABS 038-23 binds cynomolgus monkey (Macaca fascicularis) NGAL and cross-reacts with human NGAL.
ABS 038-23 binds human NGAL in complex with MMP-9.
Not determined
ABS 038-23 (as capture antibody) forms a sandwich pair with HYB 211-02 (as biotinylated detection antibody) for measuring cynomolgus monkey (Macaca fascicularis) and human NGAL.
ABS 038-23 binds human NGAL in solution.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/250 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as capture antibody. The antibody should be coated onto microwells at a minimum protein concentration of 5 µg/ml.
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-2543.
Downloads
ABS 038-23.pdf (32.84 kB) ABS 038-23 product specification sheet
Monkey_NGAL_flyer.pdf (650.73 kB)
Monkey NGAL flyer
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April 23 2008
